ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of zoledronic acid on cell cycle blocking and induction of apoptosis in lung cancer cell line 95D cells, and their mechanisms of action.</p><p><b>METHODS</b>The effect of zoledronic acid (ZOL) on proliferation of lung cancer cell line 95D cells was observed by MTT assay. Cell cycle and apoptosis of the lung cancer cells was examined by flow cytometry. The apoptosis in the cancer cells was also examined by light and transmission electron microscopy. The expressions of ERK, Bcl-2, Bax and survivin were measured by Western blot and RT-PCR.</p><p><b>RESULTS</b>ZOL showed inhibitory effect on the proliferation of lung cancer cells in vitro, in a time-dependant and a dose-dependant manner. With time extending after ZOL treatment, the number of apoptosis cells was increased. The expression of ERK, Bcl-2 and survivin was down-regulated and that of Bax up-regulated.</p><p><b>CONCLUSION</b>Zoledronic acid can block the cell cycle and induce apoptosis in lung cancer cells in vitro.</p>
Subject(s)
Humans , Antineoplastic Agents , Pharmacology , Apoptosis , Cell Cycle , Cell Line, Tumor , Diphosphonates , Pharmacology , Dose-Response Relationship, Drug , Imidazoles , Pharmacology , Inhibitor of Apoptosis Proteins , Lung Neoplasms , Metabolism , Pathology , Microtubule-Associated Proteins , Genetics , Metabolism , Mitogen-Activated Protein Kinase 1 , Metabolism , Mitogen-Activated Protein Kinase 3 , Metabolism , Proto-Oncogene Proteins c-bcl-2 , Metabolism , RNA, Messenger , Metabolism , bcl-2-Associated X Protein , Genetics , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To observe transient receptor potential melastatin 7-like (TRPM7L) expression changes post myocardial infarction (MI) in mouse cardiac fibroblast (CF).</p><p><b>METHODS</b>TRPM7 expression and Ca2+ influx in CF from MI and control mice were quantified by mRNA RT-PCR and whole cell patch clamp technique.</p><p><b>RESULTS</b>(1) TRPM7 expression was significantly upregulated post MI and Ca2+ influx of CF were significantly increased post MI [(7.4 +/- 0.7) pA/pF vs. (16.2 +/- 1.7) pA/pF, P < 0.01] and Ca2+ influx of CF increased 3-fold under lower pH condition; (2) These effects could be blocked by knock-out TRPM7 gene with SiRNA.</p><p><b>CONCLUSION</b>TRPM7L upregulation post MI and under lower pH condition are responsible for increased Ca2+ influx in CF.</p>
Subject(s)
Animals , Mice , Calcium , Metabolism , Cells, Cultured , Disease Models, Animal , Gene Expression Regulation , Myoblasts, Cardiac , Metabolism , Myocardial Infarction , Metabolism , Patch-Clamp Techniques , RNA, Messenger , Genetics , TRPM Cation Channels , MetabolismABSTRACT
Objective To investigate the effects of rehabilitation and exercise training on endothelial func- tion and collateral circulation in patients with coronary heart disease(CHD)who underwent PCI.Methods Sixty- three cases were divided into three groups:group A undertook rehabilitation protocol with three stages,group B with additional extracorporeal counterpulsation in addition to the intervention of group A,group C acted as control.The Bruce submaximal treadmill,coronary angiography and Doppler were conducted for evaluation of the functional per- formance,lateral branching and endothelium function.Results(1)The value of RPP and RPE of groups A and B were significantly higher than those of the control group as quantified by exercise test alter the third stage of rehabilita- tion(P